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Benzonase Nuclease/Binuclease
Benzonase Nuclease/Binuclease
产品编号:2604A
产品规格:50KU
产品价格:¥ 860
包装:盒
储存条件:4℃
CAS No.: |
9025-65-4 |
E.C.: |
3.1.30.2 |
Source: |
Serratia marcescens |
Cat#: |
2604A |
Activity: |
Solid enzyme ≥ 20 kU/mg lyophilized powder Liquid enzyme ≥ 250 U/μL |
Specific activity: |
≥ 1000 kU/mg protein |
Molecular weight: |
27.9 kD |
Form: |
Solid: white lyophilized powder; Liquid: clear colorless buffered aqueous glycerol solution. |
Purity: |
≥ 90%; (SDS-PAGE) |
Protease: |
None detected; |
Endotoxin: |
None detected (Tachypleus Amebocyte Lysate); |
Optimal pH: |
8.0 (operational pH 6~10); |
Optimal temperature: |
37°C (operational 0~42°C); |
Activator: |
1~10 mM Mg2+ |
Shelf life: |
Solid, 3 years (below 4°C); Liquid, 1 years (-20°C). |
Transportation and Storage:
Shipment of the liquid form is below 4°C or with blue ice. Storage at -20°C is recommended.
The solid enzyme can be shipped at room temperature. It is recommended to be stored below 4°C.
Storage buffer:
20 mM Tris-HCl (pH 8.0), 2 mM MgCl2 , 20 mM NaCl, 50% glycerol.
Dilution buffer:
20 mM Tris-HCl (pH 8.0), 2 mM MgCl2 , 20 mM NaCl.
Unit definition:
According to the protocol from Sigma-Aldrich.
One unit (U) of binuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min under the condition above.
1. Removal of nucleic acids (DNA and RNA) in the biological products.
2. Viscosity reduction in cell lysates.
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