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Human IgG1, kappa Isotype Control

Human IgG1, kappa Isotype Control

Number:91001A/91001B

Specifications:100g/25mg

Price:¥ 1500/15000

Package:package

Storage:F

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Human IgG1, kappa Isotype Control


General Information

Name   Human IgG1, kappa Isotype Control

Type:     Human Monoclonal Antibody

Isotype:   Human IgG1, kappa light chain

Clone ID:   E34

Applications:   This IgG1/kappa isotype antibody can be used as the negative antibody control in any cell based or animal based assay. For example, this antibody can be used as negative control in cell based T cells activation or ADCC assay(Please refer to Fig1 and 2 in the attachment).

Please Note:   Optimal concentrations/dilutions should be determined by the end user.

Preparation:   CHO production

Purification:   Protein A、CEX

Formulation:   0.2 μm filtered solution in 100mmol NaCl,10mmol Tri-sodium citrate dihydrate, 0.02% Tween 80,PH6.0

Storage:   This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.


QC Testing

Character

Method

Result

Antibody concentration

UV

> 5 mg/mL

Aggregation

SEC-HPLC

3% aggregation

Purity

SEC-HPLC

>95% (Fig 3)

Endotoxin

LAL gel clotting

2 EU/mg









The attachment:
 T cells activation asssy with IgG1 as negative control

Figures1:T cells activation asssy with IgG1 as negative control

ADCC assay with DP47-IgG1 as negative control
Figures2: ADCC assay with IgG1 as negative control

Purity of DP47-IgG1 by SEC-HPLC
Figures3:Purity of IgG1 by SEC-HPLC